histone h3 (Bioss)

Bioss is a verified supplier

Bioss manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Bioss histone h3
Histone H3, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+histone+h3/Histone+H3+Polyclonal+Antibody/pm41792371-256-21-27
Average 93 stars, based on 32 article reviews

histone h3 - by Bioz Stars, 2026-09

93/100 stars

Images

Western Blot:

Article Title: The myocardin-related transcription factor MKL co-regulates the cellular levels of two profilin isoforms
Article Snippet: .. Immunoblotting conditions for the various antibodies were as follows: monoclonal Pfn1 (Abcam (ab124904), Cambridge, MA, 1:3000); monoclonal GAPDH (Bio-Rad (G9545), 1:3000); monoclonal p27 Kip1 (BD Biosciences (610241), 1:2000); polyclonal MKL1 (Santa Cruz Biotechnology (sc-32909), 1:500); polyclonal MKL2 (Santa Cruz Biotechnology (sc-98989), 1:500); polyclonal SRF (Santa Cruz Biotechnology (sc-335), 1:500); polyclonal Pfn2 (Santa Cruz Biotechnology (sc-100955), 1:500); monoclonal STAT1 (Cell Signaling (9172P), Danvers, MA, 1:1000); monoclonal STAT3 (Cell Signaling (4904P), 1:1000); monoclonal LC3 (Cell Signaling (12741T), 1:1000); monoclonal tubulin (Sigma (T9026), 1:3000); polyclonal CD63 (Systems Biosciences (EXOAB-CD63A-1), 1:1000); polyclonal histone-H3 (Bioss Antibodies (bs-0349R), 1:100); and monoclonal FLAG (Sigma (F3165), 1:3000). mRNA extraction/quantitative RT-PCR Total RNA was extracted from cultured cells using the RNeasy mini kit (Qiagen) according to the manufacturer's instructions. .. Complementary DNA (cDNA) was synthesized from 1 μg of RNA using the Quantitect reverse transcription kit (Qiagen) following the manufacturer's instructions.

Article Title: The myocardin-related transcription factor MKL co-regulates the cellular levels of two profilin isoforms
Article Snippet: .. Immunoblotting conditions for the various antibodies were as follows: monoclonal Pfn1 (Abcam (ab124904), Cambridge, MA, 1:3000); monoclonal GAPDH (Bio-Rad (G9545), 1:3000); monoclonal p27 Kip1 (BD Biosciences (610241), 1:2000); polyclonal MKL1 (Santa Cruz Biotechnology (sc-32909), 1:500); polyclonal MKL2 (Santa Cruz Biotechnology (sc-98989), 1:500); polyclonal SRF (Santa Cruz Biotechnology (sc-335), 1:500); polyclonal Pfn2 (Santa Cruz Biotechnology (sc-100955), 1:500); monoclonal STAT1 (Cell Signaling (9172P), Danvers, MA, 1:1000); monoclonal STAT3 (Cell Signaling (4904P), 1:1000); monoclonal LC3 (Cell Signaling (12741T), 1:1000); monoclonal tubulin (Sigma (T9026), 1:3000); polyclonal CD63 (Systems Biosciences (EXOAB-CD63A-1), 1:1000); polyclonal histone-H3 (Bioss Antibodies (bs-0349R), 1:100); and monoclonal FLAG (Sigma (F3165), 1:3000). .. Total RNA was extracted from cultured cells using the RNeasy mini kit (Qiagen) according to the manufacturer's instructions.

Article Title: The myocardin-related transcription factor MKL co-regulates the cellular levels of two profilin isoforms
Article Snippet: .. Immunoblotting conditions for the various antibodies were: monoclonal Pfn1 (Abcam [ab124904], Cambridge, 1:3000;), monoclonal GAPDH (Biorad [G9545], Hercules, CA, 1:3000), monoclonal p27kip1 (BD Biosciences [610241], Frankin Lakes, NJ, 1:2000), polyclonal MKL1 (Santa Cruz [sc‐32909], 1:500), polyclonal MKL2 (Santa Cruz [sc‐ 98989], 1:500), polyclonal SRF (Santa Cruz [sc‐335], 1:500), polyclonal Pfn2 (Santa Cruz [sc‐100955], 1:500), monoclonal STAT1 (Cell Signaling [9172P], Danvers, MA, USA, 1:1000), monoclonal STAT3 (Cell Signaling [4904P], 1:1000), monoclonal LC3 (Cell Signaling [12741T], 1:1000), monoclonal tubulin (Sigma Aldrich [T9026], 1:3000), polyclonal CD63 (Systems Biosciences [EXOAB‐CD63A‐1], 1:1000), Polyclonal Histone H3 (Bioss Antibodies [bs-0349R], 1:100) and monoclonal Flag (Sigma Aldrich [F3165], 1:3000). mRNA Extraction/qRT-PCR Total RNA was extracted from cultured cells using the RNeasy Mini Kit (QIAGEN) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized from 1μg RNA using the Quantitect Reverse Transcription Kit (QIAGEN) following the manufacturer’s instructions.

Extraction:

Article Title: The myocardin-related transcription factor MKL co-regulates the cellular levels of two profilin isoforms
Article Snippet: .. Immunoblotting conditions for the various antibodies were: monoclonal Pfn1 (Abcam [ab124904], Cambridge, 1:3000;), monoclonal GAPDH (Biorad [G9545], Hercules, CA, 1:3000), monoclonal p27kip1 (BD Biosciences [610241], Frankin Lakes, NJ, 1:2000), polyclonal MKL1 (Santa Cruz [sc‐32909], 1:500), polyclonal MKL2 (Santa Cruz [sc‐ 98989], 1:500), polyclonal SRF (Santa Cruz [sc‐335], 1:500), polyclonal Pfn2 (Santa Cruz [sc‐100955], 1:500), monoclonal STAT1 (Cell Signaling [9172P], Danvers, MA, USA, 1:1000), monoclonal STAT3 (Cell Signaling [4904P], 1:1000), monoclonal LC3 (Cell Signaling [12741T], 1:1000), monoclonal tubulin (Sigma Aldrich [T9026], 1:3000), polyclonal CD63 (Systems Biosciences [EXOAB‐CD63A‐1], 1:1000), Polyclonal Histone H3 (Bioss Antibodies [bs-0349R], 1:100) and monoclonal Flag (Sigma Aldrich [F3165], 1:3000). mRNA Extraction/qRT-PCR Total RNA was extracted from cultured cells using the RNeasy Mini Kit (QIAGEN) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized from 1μg RNA using the Quantitect Reverse Transcription Kit (QIAGEN) following the manufacturer’s instructions.

Reverse Transcription Polymerase Chain Reaction:

Cell Culture:

Article Title: The myocardin-related transcription factor MKL co-regulates the cellular levels of two profilin isoforms
Article Snippet: .. Immunoblotting conditions for the various antibodies were: monoclonal Pfn1 (Abcam [ab124904], Cambridge, 1:3000;), monoclonal GAPDH (Biorad [G9545], Hercules, CA, 1:3000), monoclonal p27kip1 (BD Biosciences [610241], Frankin Lakes, NJ, 1:2000), polyclonal MKL1 (Santa Cruz [sc‐32909], 1:500), polyclonal MKL2 (Santa Cruz [sc‐ 98989], 1:500), polyclonal SRF (Santa Cruz [sc‐335], 1:500), polyclonal Pfn2 (Santa Cruz [sc‐100955], 1:500), monoclonal STAT1 (Cell Signaling [9172P], Danvers, MA, USA, 1:1000), monoclonal STAT3 (Cell Signaling [4904P], 1:1000), monoclonal LC3 (Cell Signaling [12741T], 1:1000), monoclonal tubulin (Sigma Aldrich [T9026], 1:3000), polyclonal CD63 (Systems Biosciences [EXOAB‐CD63A‐1], 1:1000), Polyclonal Histone H3 (Bioss Antibodies [bs-0349R], 1:100) and monoclonal Flag (Sigma Aldrich [F3165], 1:3000). mRNA Extraction/qRT-PCR Total RNA was extracted from cultured cells using the RNeasy Mini Kit (QIAGEN) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized from 1μg RNA using the Quantitect Reverse Transcription Kit (QIAGEN) following the manufacturer’s instructions.

Review